Hydroxypropyl chitosan/soy isolate protein conduits promote peripheral nerve regeneration.

Abstract

Designing scaffolds, with optimized micro-structure and function for promoting the release of neuro-related factors, is significant in peripheral nerve regeneration. Herein, a series of hydroxypropyl chitosan/soy protein isolate composite sponges (HCSS) were fabricated by a freeze-drying technique. The physicochemical properties of the resultant HCSS were examined by a Fourier infrared spectrometer, X-ray diffractometer, scanning electron microscope, water absorption assay, water retention assay, and compressive strength assay. The results indicated that HCSS exhibited an interconnected porous micro-structure and a high water retention ratio with the increase in SPI content. The biological characterization found that the HCSS-50 containing 50% SPI content profoundly promoted the proliferation of RSC96 cells and the secretion of neuro-related factors without excessive ROS production. In addition, HCSS-50 could significantly promote the expression of neuro-related factors; for example, the expression of TGF-β was 3 times higher than that of the control group. Finally, an optimized HCSS-based conduit was fabricated from HCSS-50 to repair sciatic nerve injury in rats with the combination of BMSCs or BMSC-derived Schwann cells. The results suggested that the constructed HCSS-based conduit accompanying BMSC-derived Schwann cells could effectively promote axonal regeneration and upregulate expression of neuro-related factors such as Krox20, Zeb2, and GAP43. Collectively, a newly engineered nerve conduit system was developed by incorporating HCSS-50 and BMSC-derived Schwann cells, which could be an alternative candidate for peripheral nerve regeneration.