Association between physical activity, quadriceps muscle performance and biological characteristics of very old men and women.

Abstract

The present study aimed to evaluate the association between physical activity, knee extensors (KE) performance (i.e. isometric strength and fatigability) and biological parameters (i.e. muscle structural, microvascular and metabolic properties) in healthy very old men and women. Thirty very old adults (82±1 yr, 15 women) performed an isometric quadriceps intermittent fatigue (QIF) test for the assessment of KE maximal force, total work (index of absolute performance) and fatigability. Muscle biopsies from the vastus lateralis muscle were collected to assess muscle fibers type and morphology, microvasculature and enzymes activity. Correlation analyses were used to investigate the relationships between physical activity (steps.day -1, actimetry), KE performance, and biological data for each sex separately.Men, compared to women, showed greater total work at the QIF test (44497±8629\xa0N.s vs. 26946±4707\xa0N.s; P<0.001). Steps.day -1 were correlated with total work only for women (r=0.73, P=0.011). In men, steps.day -1 were correlated with the percentage (r=0.57, P=0.033), shape factor (r=0.75, P = 0.002) and capillary tortuosity of type IIX fibers (r=0.59, P=0.035). No other relevant correlations were observed for men or women between steps.day -1 and biological parameters. Physical activity level was positively associated with the capacity of very old women to perform a fatiguing test, but not maximal force production capacity of the KE. Physical activity of very old men was not correlated with muscle performance. We suggest that very old women could be at higher risk of autonomy loss and increasing the steps.day -1 count could provide a sufficient stimulus for adaptations in less active women.