Brown Rice Inhibits Development of Nonalcoholic Fatty Liver Disease in Obese Zucker (fa/fa) Rats by Increasing Lipid Oxidation Via Activation of Retinoic Acid Synthesis.

Abstract

BACKGROUND\nWhite rice and its unrefined form, brown rice, contain numerous compounds that are beneficial to human health. However, the starch content of rice can contribute to obesity, a main risk factor for nonalcoholic fatty liver disease (NAFLD).\n\n\nOBJECTIVES\nWe investigated the effect of rice consumption on NAFLD and its underlying molecular mechanism.\n\n\nMETHODS\nWe randomly divided 7-week-old male obese Zucker (fa/fa) rats, an animal model of NAFLD, into 3 groups (n\xa0=\xa010 each) fed 1 of 3 diets for 10 weeks: a control diet (Cont; AIN-93G\xa0diet; 53% cornstarch), a white rice diet (WR; AIN-93G\xa0diet with cornstarch replaced with white rice powder), or a brown rice diet (BR; AIN-93G\xa0diet with cornstarch replaced with brown rice powder). Liver fat accumulation and gene expression related to lipid and vitamin A metabolisms, including retinoic acid (RA) signaling, were analyzed.\n\n\nRESULTS\nHepatic lipid values were significantly decreased in the BR group compared with the Cont group, by 0.4-fold (P <\xa00.05). The expression of genes related to hepatic fatty acid oxidation, such as carnitine palmitoyltransferase 2, was approximately 2.1-fold higher in the BR group than the Cont group (P <\xa00.05). The expression of peroxisomal acyl-coenzyme A oxidase 1 and acyl-CoA dehydrogenase medium chain was also significantly increased, by 1.6-fold, in the BR group compared with the Cont group (P <\xa00.05). The expression of VLDL-secretion-related genes, such as microsomal triglyceride transfer protein, was also significantly higher in the BR group (2.4-fold; P <\xa00.05). Furthermore, aldehyde dehydrogenase 1 family member A1, an RA synthase gene, was 2-fold higher in the BR group than the Cont group (P <\xa00.05).\n\n\nCONCLUSIONS\nBrown rice prevented development of NAFLD in obese Zucker (fa/fa) rats. The beneficial effects of pregelatinized rice on NAFLD could be manifested as increased fatty acid oxidation and VLDL secretion, which are regulated by RA signaling.