Rapid evaluation of the resistance of citrus germplasms against Xanthomonas citri subsp. citri.

Abstract

Xanthomonas citri subsp. citri (Xcc) is the causal agent of citrus bacterial canker (CBC), one of the most devastating citrus diseases. Most commercial citrus varieties are susceptible to CBC. However, some citrus varieties and wild citrus germplasms are CBC-resistant and are promising in genetic improvements of citrus resistance against CBC. We aimed to evaluate citrus germplasms for resistance against CBC. First, we developed a rapid evaluation method based on enhanced yellow fluorescent protein (eYFP)-labeled Xcc. The results demonstrated that eYFP does not affect the growth and virulence of Xcc. Xcc-eYFP allows measuring of bacterial titers, but is more efficient and rapid than the plate colony counting method. Next, we evaluated citrus germplasms collected in China. Based on symptoms and bacterial titers, we identified that two citrus germplasms ( Ichang papeda, and Huapi kumquat) are resistant, whereas eight citrus germplasms ( Chongyi wild mandarin, Mangshan wild mandarin, Ledong kumquat, Dali citron, Yiliang citron, Longyan kumquat, Bawang kumquat and Daoxian wild mandarin) are tolerant. In summary, we have developed a rapid evaluation method to test the resistance of citrus plants against CBC. This method was successfully used to identify two highly canker-resistant citrus germplasms and eight citrus germplasms with canker tolerance. These results could be leveraged in traditional breeding contexts or be used to identify canker resistance genes to improve the disease resistance of commercial citrus varieties via biotechnological approaches.