The histological microstructure and in vitro mechanical properties of the human female postmenopausal perineal body

Abstract

Objective: The perineal body connects muscles from the pelvic floor and is critical for support of the lower part of the vagina and proper function of the anal canal. We determined mechanical parameters and volume fractions of main components of the human female postmenopausal perineal body. Methods: The specimens were taken from 15 fresh female cadavers (age 74\u200a±\u200a10, mean\u200a±\u200astandard deviation). Seventy-five specimens from five regions of the perineal body were processed histologically to assess volume fractions of tissue components using stereological point testing grid. Fifteen specimens taken from the midline region were loaded uniaxially with 6\u200amm/min velocity until tissue rupture to determine Young s modulus of elasticity, ultimate stresses, and strains. Results: The perineal body was composed of collagen (29%), adipose cells (27%), elastin (7%), smooth muscle (11%), and skeletal muscle (3%). The residual tissue (19%) constituted mostly peripheral nerves, lumina of blood vessels, fibroblasts, and fibrocytes. Young s modulus of elasticity at midline region was 18\u200akPa (median) at small and 232\u200akPa at large deformations, respectively. The ultimate stress was 172\u200akPa and the ultimate strain was 1.4. Conclusions: We determined the structural and mechanical parameters of the perineal body. The resultant data could be used as input for models simulating pelvic floor prolapse or dysfunction.