Chitinophaga silvisoli sp. nov., isolated from forest soil.

Abstract

A Gram-stain-negative, rod-shaped and aerobic bacterium, designated K20C18050901T, was isolated from forest soil collected on 11 September 2017 from Dinghushan Biosphere Reserve, Guangdong Province, PR China (23° 10 24 N; 112° 32 10 E). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain K20C18050901T belongs to the genus Chitinophaga, and showed the highest similarities to Chitinophaga sancti NBRC 15057T (98.6\u200a%) and Chitinophaga oryziterrae JCM 16595T (96.9\u200a%). The major fatty acids (>10\u200a%) were iso-C15\u200a:\u200a0, C16\u200a:\u200a1ω5c, summed feature 3 (C16\u200a:\u200a1ω6c and/or C16\u200a:\u200a1ω7c) and iso-C17\u200a:\u200a0 3-OH. The predominant respiratory quinone was menaquinone-7. The major polar lipid was phosphatidylethanolamine. The draft genome size of strain K20C18050901T was 8.36\u2009Mb with a DNA G+C\u2009content of 44.7 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between strain K20C18050901T and C. sancti NBRC 15057T were 31.40 and 85.82\u200a%, respectively. On the basis of phenotypic, genotypic and phylogenetic analysis, strain K20C18050901T represents a novel species of the genus Chitinophaga, for which the name Chitinophagasilvisoli sp. nov. is proposed. The type strain is K20C18050901T (=GDMCC 1.1411T=KCTC 62860T).