Dyella solisilvae sp. nov., isolated from mixed pine and broad-leaved forest soil.

Abstract

A Gram-stain-negative, aerobic, motile, yellow-pigmented, rod-shaped with a single polar flagellum bacterial strain, designated strain DHG54T, was isolated from a forest soil sample of Dinghushan Biosphere Reserve, Guangdong Province, China. Strain DHG54T grew at 12-37\u2009°C (optimum, 28\u2009°C), pH 4.5-8.0 (optimum, pH 6.0-7.0) and in the presence of 0-3.0\u200a% (w/v) NaCl (optimum, 0-1.5\u200a%, w/v). Based on 16S rRNA gene sequence analysis, strain DHG54T formed a clade with the members of the genus Dyella and showed highest sequence similarities of 98.2\u200a% to Dyella japonica DSM 16301T and Dyella terrae KACC 12748T. This was also supported by phylogenetic analysis based on the concatenated partial gyrB, lepA and recA housekeeping gene sequences. DNA-DNA hybridization results between strain DHG54T and closely related Dyella species were all lower than 70\u200a%. Ubiquinone-8 was the only respiratory quinone, and iso-C15\u200a:\u200a0, iso-C17\u200a:\u200a0 and iso-C17\u200a:\u200a1\u2009ω9c were major fatty acids. The DNA G+C\u2009content of strain DHG54T was 65.4\u2009mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of the polyphasic characterization results presented here, strain DHG54T represents a novel species of the genus Dyella, for which the name Dyellasolisilvae sp. nov. (type strain DHG54T=GDMCC 1.1187T = LMG 30091T) is proposed.