Paraburkholderia telluris sp. nov., isolated from subtropical forest soil.

Abstract

Strain DHOC27T is a Gram-stain-negative, aerobic, non-motile, light yellow-pigmented and rod-shaped bacterium isolated from the forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China. It grew at 4-37\u2009°C (optimal 28-33\u2009°C), pH 4.0-8.5 (optimal 4.5-6.0) and 0-1.5 (optimal 0-0.5)\u200a% (w/v) NaCl. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a clade with Paraburkholderia phenazinium LMG 2247T, Paraburkholderia. sartisoli LMG 24000T and Paraburkholderia. pallidirosea DHOK13T, with a sequence similarity of 98.5, 97.5 and 98.1\u200a% to the above strains, respectively. The DNA G+C\u2009content of DHOC27T was 62.3\u2009mol%. The digital DNA-DNA relatedness values and the average nucleotide identities between strain DHOC27T and P. phenazinium LMG 2247T and P. sartisoli LMG 24000T were 26.9 and 24.3\u200a% and 82.3 and 79.9\u200a%, respectively. C16\u200a:\u200a0, C17\u200a:\u200a0 cyclo and C19\u200a:\u200a0 cyclo ω8c were the major fatty acids, and ubiquinone-8 was the major respiratory quinone detected, all of which supported the affiliation of DHOC27T to the genus Paraburkholderia. On the basis of the data presented above, strain DHOC27T represents a novel species of the genus Paraburkholderia and the name Paraburkholderia telluris sp. nov. is proposed. The type strain is DHOC27T (=LMG 30263T=GDMCC 1.1281T).