Paraburkholderia dinghuensis sp. nov., isolated from soil.

Abstract

A novel Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterial strain, designated DHOA04T, was isolated from a forest soil sample collected at Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31 E 23° 10 N). It grew optimally at 28-33\u2009°C\u2009and pH 6.5-7.0. Strain DHOA04T contained Q-8 as the major respiratory quinone. Its main fatty acids were C16\u200a:\u200a0, C17\u200a:\u200a0cyclo, summed feature 3 (C16\u200a:\u200a1ω6c and/or C16\u200a:\u200a1ω7c) and summed feature 8 (C18\u200a:\u200a1ω7c and/or C18\u200a:\u200a1ω6c). The DNA G+C\u2009content of DHOA04T was 63.0\u2009mol%, which is in the range of the genus Paraburkholderia. The average nucleotide identity and digital DNA-DNA hybridization values for the complete genomes were 81.6-83.0 and 25.5-27.0\u200a% between strain DHOA04T and five closely related type strains. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified aminophospholipids. On the basis of 16S rRNA gene sequence analysis, the strain was found to be closely related to members of the genus Paraburkholderia, but clearly separated from the established species. Phylogenetic analysis based on the 16S rRNA gene sequences using the maximum-likelihood algorithm indicated that strain DHOA04T was most closely related to Paraburkholderia ferrariae NBRC 106233T. The phenotypic, chemotaxonomic and phylogenetic data, and genome analysis showed that strain DHOA04T represents a novel species of the genus Paraburkholderia, for which the name Paraburkholderia dinghuensis sp. nov. is proposed. The type strain is DHOA04T (=KCTC 42627T=LMG 28839T).