Deminuibacter soli gen. nov., sp. nov., isolated from forest soil, and reclassification of Filimonas aurantiibacter as Arvibacter aurantiibacter comb. nov.

Abstract

A novel strain, designated K23C18032701T, was isolated from a sample of forest soil collected from Dinghushan Biosphere Reserve, Guangdong Province, PR China. The strain was Gram-stain-negative, aerobic, motile and showed a shape change from a filamentous cell to coccobacilli. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strain belongs to the family Chitinophagaceae, and showed the highest similarities to Arvibacter flaviflagrans JCM 31293T (95.0\u200a%) and Filimonas aurantiibacter LMG 29039T (94.4\u200a%). The major cellular fatty acids included iso-C15\u200a:\u200a0, iso-C17\u200a:\u200a0 3-OH and iso-C15\u200a:\u200a1 G. The predominant polar lipid was phosphatidylethanolamine (PE). The predominant respiratory quinone was menaquinone-7. The major polyamine was sym-homospermidine. The draft genome size of strain K23C18032701T was 5.84\u2009Mb with a DNA G+C\u2009content of 47.2\u2009mol%. Based on phenotypic, genotypic and phylogenetic analysis, strain K23C18032701T represents a novel species of a new genus in the family Chitinophagaceae, for which the name Deminuibacter soli is proposed. The type strain is K23C18032701T (=GDMCC 1.1403T=KCTC 62913T). We also propose the reclassification of Filimonas aurantiibacter as Arvibacter aurantiibacter comb. nov. (type strain 1458T=NRRL B-65305T=LMG 29039T).