International journal of systematic and evolutionary microbiology | 2019
Leucobacter muris sp. nov., isolated from the nose of a laboratory mouse.
Abstract
A Gram-stain-positive, rod-shaped, aerobic, non-motile, white, opaque bacterial isolate, designated 924/12T, was isolated from the nose of a laboratory mouse in Düsseldorf, Germany. The 16S rRNA gene sequence analyses indicated the phylogenetic position of the strain within the genus Leucobacter. Similarity levels over 97\u200a% were recorded between the 16S rRNA gene sequence of strain 924/12T and the type strains of the species Leucobacter chironomi DSM 19883T (99.5\u200a%), followed by Leucobacter celersubsp. astrifaciens CBX151T (97.6\u200a%), Leucobacter celersubsp. celer NAL101T (97.5\u200a%), Leucobacter kyeonggiensis F3-P9 (97.5\u200a%), Leucobacter zeae CC-MF41T (97.3\u200a%), Leucobacter chromiiresistens JG31T (97.1\u200a%), Leucobacter triazinivorans JW-1T (97.1\u200a%), Leucobacter corticis 2\u2009C-7T (97.0\u200a%) and Leucobacter aridicolis CIP108388T (97.0\u200a%). DNA-DNA hybridization and whole genomic comparison, mandatory to taxonomically separate strain 924/12T from the type strain of L. chironomi, revealed similarity values of 40.4 and 30.8\u200a%, respectively, thus below the threshold of 70\u200a% recommended differentiating between species. The cell-wall amino acids of the novel isolate were diaminobutyric acid, alanine, glycine, threonine and glutamic acid. The major fatty acids were anteiso-C15\u200a:\u200a0, anteiso-C17\u200a:\u200a0 and iso-C16\u200a:\u200a0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unknown lipid, whereas the predominant menaquinones were MK-11 and MK-10. The genomic DNA G+C\u2009content of strain 924/12T was 70.6\u2009mol%. Phylogenetic analyses based on the 16S rRNA gene sequences and the phenotypical differences between strain 924/12T and the other closely related type strains of the genus Leucobacter indicated that strain 924/12T represents a novel species within the genus Leucobacter, family Microbacteriaceae, for which the name Leucobacter muris sp. nov. is proposed. The type strain is 924/12T (=DSM 101948T=CCM 8761T).