Pontibacter flavimaris sp. nov., of the family Hymenobacteraceae, isolated from marine sediment.

Abstract

A Gram-stain-negative, ovoid or rod-shaped, non-flagellated, motile-by-gliding and aerobic bacteria, designated S10-8T, was isolated from marine sediment of the Yellow Sea. Colonies of strain S10-8T had a pink-red pigmentation and its cells were approximately 0.5-0.8 μm×1.0-2.5 μm in size. Growth occurred at 10-45 °C (optimally at 33-37 °C), in the presence of 0-12.0\u200a% NaCl (optimally at 2.0-5.0\u200a%, w/v) and at pH 5.0-8.5 (optimally at pH 7.0-7.5). Phylogenetic analysis of the 16S rRNA gene indicated that strain S10-8T is a member of the genus Pontibacter within the family Hymenobacteraceae, and the 16S rRNA gene sequence similarity of strain S10-8T to its closest relative Pontibacter actiniarum KCTC 12367T was 96.9\u200a%. Strain S10-8T contained MK-7 as the predominant menaquinone and summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B) and iso-C15:0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified lipid. The size of the draft genome was 4\u200a623\u200a791 bp and the G+C content was 53.5 mol%. There were low DNA-DNA hybridization values (<48.3±5.2\u200a%) and average nucleotide identity values (<86.5\u200a%) between strain S10-8T and the most closely related recognized Pontibacter species. Therefore, we propose a novel species in the genus Pontibacter to accommodate the novel isolate: Pontibacter flavimaris sp. nov. (type strain S10-8T=KCTC 42769T=ACCC 19859T).