Measuring splash-dispersal of a major wheat pathogen in the field

Abstract

Capacity for dispersal is a fundamental fitness component of plant pathogens. Empirical characterization of plant pathogen dispersal is of prime importance for understanding how plant pathogen populations change in time and space. We measured dispersal of Zymoseptoria tritici in natural environment. Primary disease gradients were produced by rain-splash driven dispersal and subsequent transmission via asexual pycnidiospores from infected source. To achieve this, we inoculated field plots of wheat (Triticum aestivum) with two distinct Z. tritici strains and a 50/50 mixture of the two strains. We measured effective dispersal of the Z. tritici population based on pycnidia counts using automated image analysis. The data were analyzed using a spatially-explicit mathematical model that takes into account the spatial extent of the source. We employed robust bootstrapping methods for statistical testing and adopted a two-dimensional hypotheses test based on the kernel density estimation of the bootstrap distribution of parameter values. Genotyping of re-isolated pathogen strains with strain-specific PCR-reaction further confirmed the conclusions drawn from the phenotypic data. The methodology presented here can be applied to other plant pathosystems. We achieved the first estimates of the dispersal kernel of the pathogen in field conditions. The characteristic spatial scale of dispersal is tens of centimeters – consistent with previous studies in controlled conditions. Our estimation of the dispersal kernel can be used to parameterize epidemiological models that describe spatial-temporal disease dynamics within individual wheat fields. The results have the potential to inform spatially targeted control of crop diseases in the context of precision agriculture.