A systematic review and meta-analysis of the potential non-human animal reservoirs and arthropod vectors of the Mayaro virus

Abstract

Improving our understanding of Mayaro virus (MAYV) ecology is critical to guide surveillance and risk assessment. We conducted a PRISMA-adherent systematic review of the published and grey literature to identify potential arthropod vectors and non-human animal reservoirs of MAYV. We searched PubMed, Embase, Web of Science, SciELO and grey-literature sources including PAHO databases and dissertation repositories. Studies were included if they assessed MAYV virological/immunological measured occurrence in field-caught, domestic, or sentinel animals or in field-caught arthropods. We conducted an animal seroprevalence meta-analysis using a random effects model. We compiled granular georeferenced maps of non-human MAYV occurrence and graded the quality of the studies using a customized framework. Overall, 57 studies were eligible out of 1523 screened, published between the years 1961 and 2020. Seventeen studies reported MAYV positivity in wild mammals, birds, or reptiles and five studies reported MAYV positivity in domestic animals. MAYV positivity was reported in 12 orders of wild-caught vertebrates, most frequently in the orders Charadriiformes and Primate. Sixteen studies detected MAYV in wild-caught mosquito genera including Haemagogus, Aedes, Culex, Psorophora, Coquillettidia, and Sabethes. Vertebrate animals or arthropods with MAYV were detected in Brazil, Panama, Peru, French Guiana, Colombia, Trinidad, Venezuela, Argentina, and Paraguay. Among non-human vertebrates, the Primate order had the highest pooled prevalence (PP) at 13.1% (95% CI: 4.3-25.1%). From the three most studied primate genera we found the highest prevalence was in Alouatta (PP: 32.2%, 95% CI: 0.0-79.2%), followed by Callithrix (PP: 17.8%, 95% CI: 8.6-28.5%), and Cebus/Sapajus (PP: 3.7%, 95% CI: 0.0-11.1%). We further found that MAYV occurs in a wide range of vectors beyond Haemagogus spp. The quality of evidence behind these findings was variable and prompts calls for standardization of reporting of arbovirus occurrence. These findings support further risk emergence prediction, guide field surveillance efforts, and prompt further in-vivo studies to better define the ecological drivers of MAYV maintenance and potential for emergence. Author Summary Mayaro virus (MAYV) is an emerging tropical public health threat in the Americas. We conducted a georeferenced, quality-graded systematic review to evaluate the current evidence regarding MAYV occurrence in non-human vertebrates and arthropods. Overall, 57 studies were eligible out of 1523 screened, published between the years 1961 and 2020. Seventeen studies reported MAYV positivity in wild mammals, birds, or reptiles and five studies reported MAYV positivity in domestic animals. MAYV positivity was reported in 12 orders of wild-caught vertebrates, most frequently in the orders Charadriiformes and Primate. Our systematic review identified 12 orders of wild-caught vertebrates and seven mosquito genera with evidence of MAYV occurrence. Primates had the highest pooled MAYV prevalence according to a seroprevalence meta-analysis. The graded quality of evidence behind these findings was variable and prompts calls for standardization of reporting of MAYV and perhaps other emerging arbovirus occurrence in animals and vectors. This study provides important information for public health authorities and disease ecologists concerned with the growing threat of MAYV in Latin America. Our analysis provides a foundation for future laboratory and field studies focused on the MAYV transmission cycle.