Electron cryo-microscopy at 100 and 200\u2005kV: from screening to structures

Abstract

Electron cryo-microscopy (cryo-EM) single particle analysis (SPA) method has become one of the dominating methods for high resolution structure determination of a wide variety of biological macromolecules. Such high resolution structures facilitate understanding of their functions, mechanism of action and protein ligand/drug interactions. With an increase in the popularity of cryoEM, the need for accessibility, ease of use and improved efficiency has also increased. Here we describe two cryo-transmission electron microscopes (cryo-TEMs), that are equivalent to home source X-ray diffractometers, but for cryo-EM.