Development of graphene oxide-based fluorescent sensing nanoplatform for microRNA-10b detection

Abstract

Herein, we develop a fluorescent sensor for simple detection of miRNA-10b, which combines the fluorescence quenching ability of graphene oxide (GO) and the duplex-specific nuclease (DSN) mediated target recycling amplification. Our sensor exhibits desirable sensitivity for miRNA-10b with a 530 fM detection limit that could be achieved within 75 min. Furthermore, our sensor showed good selectivity for discriminating target miRNA and other microRNAs.