Real-Time Fermentation Monitoring of Synthetic Beer Wort Using Etched Fiber Bragg Grating

Abstract

A refractometer based on an etched fiber Bragg gratings (EFBGs) sensor has been developed for monitoring beer fermentation in real time. The sensor was calibrated using synthetic solutions composed of water, maltose, and ethanol with alcohol by volume (ABV) ranging from 4.0% to 12.0% and Brix (sugar content) varying from 5% to 25%. Calibration results showed that the EFBG sensor responds with high sensitivity even to minor density variations less than 0.5 Brix ± 0.03 Brix in a quasi-linear monotonic fashion with respect to sugar and ethanol concentration, which allowed fitting an empirical model with good adherence to experimental data (low model variance) and with well-defined parameters (parameters covariance matrix with elements exhibiting small absolute values). Finally, the developed sensor was tested in a typical fermentation scenario. A rigorous material balance framework was established to relate the fit calibration model to actual density and alcohol content in real time, which is of assistance for process control strategies. As the proposed sensor can be fit in fermentation vessels for real-time (online) fermentation monitoring, it becomes a technological improvement to traditional offline measurements in terms of both biosafety and process control.