Evaluation of the Binding of PD-1 Antibody and Antigen Using Nano-Sensors

Abstract

In this paper, solid-state nanopore as a single molecule method is used to detect the binding of PD-1 antibody and antigen. Using PD-1 antibody modified nanopore, the antibody-antigen complexes are detected and distinguished with PD-1 protein and its antibody through the relative current drop analysis. The binding rate of the complexes are found changed with variation of binding temperature. And in the future, the nanopore technology may be a rapid and label-free way for patients and doctors to evaluate the drugs’ efficiency.