Physiological responses to light explain competition and facilitation in a tree diversity experiment

Abstract

Abstract Ecologists often invoke interspecific facilitation to help explain positive biodiversity-ecosystem function relationships in plant communities, but seldom test how it occurs. One mechanism through which one species may facilitate another is by ameliorating abiotic stress. Physiological experiments show that a chronic excess of light can cause stress that depresses carbon assimilation. If shading by a plant’s neighbors reduces light stress enough, it may facilitate that plant’s growth. If light is instead most often a limiting factor for photosynthesis, shading may have an adverse, competitive effect. In a temperate tree diversity experiment, we measured stem growth rates and photosynthetic physiology in broadleaf trees across a gradient of light availability imposed by their neighbors. At the extremes, trees experienced nearly full sun (monoculture), or were shaded by nearby fast-growing conifers (shaded biculture). Most species had slower growth rates with larger neighbors, implying a net competitive effect. On the other hand, the two most shade-tolerant species (Tilia americana and Acer negundo) and the most shade-intolerant one (Betula papyrifera) had faster stem growth rates with larger neighbors. The two most shade-tolerant species had large increases in photoinhibition (reduced dark-acclimated Fv/Fm) across the gradient of increasing light availability, which suggests they are more vulnerable to chronic light stress. While most species had lower carbon assimilation rates in the shaded biculture treatment, T. americana had rates up to 25% higher. T. americana also dropped its leaves 3-4 weeks earlier in monocultures, curtailing its growing season. We conclude that although large neighbors can cause light limitation in shade-intolerant species, they can also increase growth through abiotic stress amelioration in shade-tolerant species. Finally, in shade-intolerant B. papyrifera, we find a pattern of stem elongation in trees with larger neighbors, which may suggest that a shade avoidance response accounts for the apparent positive trend in stem volume. Synthesis: Both positive and negative species interactions in our experiment can be explained in large part by the photosynthetic responses of trees to the light environment created by their neighbors. We show that photosynthetic physiology can help explain the species interactions that underlie biodiversity-ecosystem function relationships. The insights that ecologists gain by searching for such physiological mechanisms may help us forecast species interactions under environmental change.