Non-invasive monitoring of saliva can be used to identify oxidative stress biomarkers in preterm and term newborns infants.

Abstract

AIM\nThe aim of this study was to appraise the feasibility and reproducibility of applying a validated analytical method to determine salivary oxidative stress biomarkers in newborn infants.\n\n\nMETHODS\nProspective observational single-center study was carried out in level III neonatal intensive care unit. Eligible patients were preterm infants and healthy full-term newborn infants. Salivary samples were analyzed in the chromatographic system.\n\n\nRESULTS\nA total of 23 premature newborn infants and 13 full-term newborns were included. We analyzed salivary levels of oxidative stress biomarkers for 5-F2t isoprostonae, 15-E2t isoprostane, prostaglandine E2 and prostaglandine F2α. The multivariate predictive model showed a positive association between female and 5-F2t isoprostonae, and between female sex and prostglandine F2α. In addition, we found a positive association between gestational age and levels of prostaglandine E2 . Furthermore, in the premature group we found a positive association between the inspired fraction of oxygen and levels of prostaglandine G2 .\n\n\nCONCLUSION\nwe identified and determined lipid peroxidation biomarkers in term and preterm newborn infants saliva using specific and validated mass spectrometry technology.