The effect of ozone therapy in root canal disinfection: a systematic review.

Abstract

AIM\nTo answer the following focused question: As regards microorganism load reduction for patients undergoing root canal treatment, is the use of ozone therapy comparable to conventional chemomechanical techniques using sodium hypochlorite (NaOCl)? .\n\n\nDATA SOURCES\nA systematic review was conducted using controlled vocabulary and free-text key words in the following databases: PubMed, Science Direct, Scopus, Web of Science and Open Grey until November 2nd, 2018. Additional studies were sought through hand searching of endodontic journals.\n\n\nSTUDY ELIGIBILITY CRITERIA, PARTICIPANTS AND INTERVENTIONS\nThe inclusion criteria comprised studies that compared microbial reduction in root canals after treatments with ozone and NaOCl in extracted mature human teeth or randomized clinical trials.\n\n\nSTUDY APPRAISAL AND SYNTHESIS METHODS\nThe quality assessment of included laboratory studies was performed with the following parameters: (1) Sample size calculation, (2) Samples with similar dimensions, (3) Control group, (4) Standardization of procedures, (5) Statistical analysis and (6) Other risk of bias. For randomized clinical trials, the qualitative analysis of the studies was performed from the bias risk assessment using the tool Bias Risk Assessment of Randomized Controlled Studies Cochrane Handbook 5.0.2.\n\n\nRESULTS\nThe search resulted in 180 published studies. After removal of duplicate studies and full text analysis, 8 studies were selected and 7 were considered low risk of bias (7 ex vivo studies and 1 random clinical trial). Overall the results demonstrated that ozone therapy provides significantly less microbial load reduction significantly lower than NaOCl. As an adjunct in chemomechanical preparation, ozone was ineffective in increasing the antimicrobial effect of NaOCl. Ozone performance was strongly associated with the application protocol used: it is dose-, time- and bacterial strain-dependent, besides the correlation with the use of complementary disinfection sources.\n\n\nLIMITATIONS\nA restricted number of randomized clinical trial was found and the difference among the methodology of the studies did not allow a meta-analysis to be performed.\n\n\nCONCLUSIONS AND IMPLICATIONS OF KEY FINDINGS\nAlthough the selected studies had limitations, this review reached a satisfactory methodological and moderate evidence quality contributing to preliminary important information regarding ozone therapy. As regards load reduction of microorganisms for patients undergoing root canal treatment, ozone is not indicated neither to replace or to complement the antimicrobial action of NaOCl.