Association of novel stop‐gained leukaemia inhibitory factor receptor gene (rs121912501) variant, leukaemia inhibitory factor and ovarian steroids with unexplained infertility among Pakistani women

Abstract

Embryo implantation is a complex process that requires sequential steps at the interface of embryo interaction with decidual endometrium. Many women after experiencing multiple attempts of assisted reproductive techniques fail to get implantation because of instability of leukaemia inhibitory factor and leukaemia inhibitory factor receptor‐signal transducer and activator of transcription factor 3 (LIF‐LIFR STAT3) signalling cascade. Therefore, this study explores the association of ovarian steroids, LIF and LIFR stop‐gained variant using the tetra primer amplification refractory mutation system‐polymerase chain reaction (TARMS‐PCR) with unexplained infertility (UEX‐IF) among Pakistani women.