Cholinergic Signaling Mechanisms and Early Implant Healing Phases in Healthy Versus Generalized Aggressive Periodontitis Patients: A prospective, case-control study.

Abstract

AIMS\nPeriodontal diseases negatively affect implant osseointegration. Perturbations in non-neuronal cholinergic signaling mechanisms are associated with periodontitis; however, their role in generalized aggressive periodontitis (GAgP) is unknown. The aim of this prospective case-control study was to determine the relationship between non-neuronal cholinergic signaling mechanisms, Secreted Ly-6/uPAR-related protein-1 (SLURP-1), Interleukin-17 (IL-17) family cytokines and healing of dental implants in health and GAgP.\n\n\nMATERIALS AND METHODS\nThirteen GAgP patients and seven periodontally healthy individuals (PH) were recruited. Peri-implant crevicular fluid (PICF) was obtained at baseline and 1-month post-placement. Acetylcholine (ACh) levels and cholinesterase activity were determined biochemically. SLURP-1, IL-17A and IL-17E levels were determined by ELISA. Marginal bone loss (MBL) at 1- and 6-month(s) post-placement was determined radiographically.\n\n\nRESULTS\nThe concentration of ACh, cholinesterase activity and IL-17A levels were elevated in PICF of patients with GAgP compared to PH individuals at baseline and 1-month post-placement. The concentration of ACh and cholinesterase activity levels in PICF correlated with levels of IL-17A and MBL around implants 1-month post-placement in patients with GAgP.\n\n\nCONCLUSIONS\nNon-neuronal cholinergic mechanisms may play a role in the aetiopathogenesis of GAgP and may directly or indirectly, through modulation of IL-17A, influence early implant osseointegration and potential long-term implant survival. This article is protected by copyright. All rights reserved.