An Agrobacterium‐delivered CRISPR/Cas9 system for targeted mutagenesis in sorghum

Abstract

Clustered regularly interspaced short palindromic repeat/CRISPR-associated Cas9 (CRISPR/Cas9) systems of bacteria and archaea have engineered for genome editing in eukaryotic genomes. In such CRISPR/Cas9 system, CRISPR RNA (crRNA) and trans-activating CRISPR RNA (tracrRNA) were engineered into a simplified single guide RNA (sgRNA). Cas9 and sgRNA form a complex that scans through genome for the protospacer adjacent motif (PAM) sequence (predominantly 5 -NGG-3 ) and for the sequence (ca. 18-20 nucleotides) complementary to the sgRNA, leading to double stranded DNA breaks (DSBs) that are exploited for site-specific DNA alterations (Jinek et\xa0al., 2012). This article is protected by copyright. All rights reserved.