Microbiology Resource Announcements | 2021
Genome Sequence and Methylation Pattern of Haloterrigena salifodinae BOL5-1, an Extremely Halophilic Archaeon from a Bolivian Salt Mine
Abstract
The halophilic archaeon Haloterrigena salifodinae BOL5-1 was isolated from a Bolivian salt mine and sequenced using single-molecule real-time sequencing. The GC-rich genome was 5.1\u2009Mbp, with a 4.2-Mbp chromosome and 5 plasmids ranging from 96 to 281\u2009kbp. The genome annotation was incorporated into HaloWeb (https://halo.umbc.edu) and the methylation patterns into REBASE (http://rebase.neb.com). Halophilic microbes capable of surviving extreme conditions are of interest for biotechnology and astrobiology (1–12). Our recent focus has been on high elevation and subsurface hypersaline environments, which yield polyextremophilic varieties. In this announcement, isolation of an extremely halophilic archaeon, Haloterrigena salifodinae BOL5-1, is reported, together with the first complete genome sequence for this species. ABSTRACT The halophilic archaeon Haloterrigena salifodinae BOL5-1 was isolated from a Bolivian salt mine and sequenced using single-molecule real-time sequencing. The GC-rich genome was 5.1\u2009Mbp, with a 4.2-Mbp chromosome and 5 plasmids ranging from 96 to 281\u2009kbp. The genome annotation was incorporated into HaloWeb (https://halo.umbc.edu), and the methylation patterns were incorporated into REBASE (http://tools.neb.com/genomes/view.php?seq_id=99167&list=1).