РОЛЬ БЕЛКА Cbx7 В ПРОЦЕССАХ РЕГЕНЕРАЦИИ ПОВРЕЖДЕННОГО ЭПИДЕРМИСА

Abstract

The understanding of alterations of epigenetic mechanisms in the development of many disorders, such as impaired wound healing, cancer and aging is increasingly seen as a priority in biomedical research. This project aimed to investigate the expression of Polycomb group protein Cbx7 in the wound epithelium and its role in the migration of keratinocytes after wounding. Cbx7 expression was determined in mouse wound samples collected at early stages of wound healing and analyzed using PCR and immunofluorescent detection methods. For cell migration analysis, HaCaT cell line was treated with a specific small molecule inhibitor MS37452 followed by the scratch assay. Analyses of Cbx7 expression revealed a significant downregulation of Cbx7 mRNA and protein levels in the wound epithelium in comparison to the unwounded or adjacent epidermis. Inhibition of Cbx7 using MS37452 showed a significant acceleration of cell migration in vitro. These data suggest that a decrease in Cbx7 is necessary for the increased epidermal keratinocytes migration immediately after skin injury. The results of this work could be useful for the development of new therapeutic approaches for improvement of skin wound healing.