ВЛИЯНИЕ ПРЕНАТАЛЬНОГО СТРЕССА НА ФОРМИРОВАНИЕ ОРЕКСИНЕРГИЧЕСКОЙ СИСТЕМЫ ГИПОТАЛАМУСА КРЫСЫ

Abstract

The influence of prenatal stress (PNS) on the development of the orexinergic and dopaminergic systems of the brain in 14 and 30-day-old Wistar rats were studied. \xa0Pups were born by females, which E13-E19 were subjected to sleep deprivation (6 hours a day) by «small platforms» method. In P14 PNS pups the violations of locomotion development were revealed by the «open field» test. In the hypothalamus a significant increase in orexin A in the periphornical area neurons was detected on the brain slices by immunohistochemical method. A high performance liquid chromatography showed the increase of dopamine level and a rate of its metabolism vs. control pups born from intact females. The results of Western-blotting demonstrate in striatum the decrease of enzyme of GABA synthesis (GAD65) and increase of level of phosphorylated tyrosine hydroxylase (serin-40) – enzyme of dopamine synthesis vs. control. In P30 the locomotion activity of PNS pups do not differ from the control, but the PNS pups are characterized by a higher level of anxiety and lower behavior. The results of morphological and biochemical studies also indicate the absence of differences in the studied parameters in the hypothalamus and in the striatum in P30 PNS pups vs. the control. The data is confirmed by the results of the electrophysiological study, which also demonstrates the absence of differences in the organization of the wakefulness-sleep cycle in the P30 PNS and control pups. The article discusses the morphofunctional interactions of the orexinergic and dopaminergic systems of the brain in the early postnatal period of the organism development and the role of orexins in the compensatory mechanisms of the brain. \nKey words: brain, hypothalamus, striatum, ontogenesis, prenatal stress, orexin, dopamine, locomotion activity, wakefulness-sleep cycle.