СТРУКТУРОСПЕЦИФИЧНЫЕ ИЗМЕНЕНИЯ СОДЕРЖАНИЯ МОНОАМИНОВ В МОЗГЕ ПРИ ОСТРОМ СТРЕССЕ, ВЫЗВАННОМ ОДНОКРАТНЫМ ВВЕДЕНИЕМ ФИЗИОЛОГИЧЕСКОГО РАСТВОРА

Abstract

Intraperitoneal (ip) administration is widely used in the experimental neurophysiology to systemically deliver the pharmacologically active substances. However, this technique involves a short-term immobilization of an animal and is accompanied by pain, therefore, it may be stressful. Here, we evaluate the neurochemical and hormonal changes that accompany the ip saline injection in rodents. We show that the acute ip saline injection leads to the increase in the corticosterone secretion in C57Bl/6N mice, which confirms the stressfulness of the procedure. Acute ip saline injection in in C57Bl/6N mice resulted in the increased dopamine turnover in the frontal cortex, hippocampus, and caudate putamen, as well as the decreased norepinephrine content in the hippocampus and hypothalamus. In Wistar rats the ip saline injection was followed by the increase in the ratio between the total dopamine metabolite content and in the total dopamine content. The observed increase of the dopaminergic system activity after an acute i.p. saline injection may affect the cognitive functions and the performance of the reward system in rodents. The experimental studies using ip drug administration and aiming at exploration of learning, memory, attention, and reinforcement, should take into account possible changes in monoaminergic transmission in the cortex and nucleus accumbens.