SAT0069\u2005PROTECTIVE EFFECT OF PROTOCATECHUIC ACID RICH FRACTION OF TRIANTHEMA PORTULACASTRUM AGAINST COLLAGEN INDUCED RHEUMATOID ARTHRITIS VIA GUT MICROBIOTA MODULATION

Abstract

Background Rheumatoid arthritis, a chronic inflammatory joint disease affects people all over the world. Trianthema portulacastrum (TP) has been traditionally utilized for the treatment of rheumatism. Objectives The current study was envisaged to evaluate the effect of protocatechuic acid rich fraction of TP on type II collagen induced rheumatoid arthritis, inflammation and microbial population of gut by using rodent model. Methods Hydroethanolic extract of TP was fractionated with three different solvents (ethyl acetate, chloroform and n-butanol) and quantitatively analyzed with HPLC-DAD method which revealed n-butanol fraction of TP (BFTP) is rich in protocatechuic acid along with other phenolic compounds. Animals were randomly divided into five groups. Four groups were injected with bovine type-II collagen (BTC-II; 100μg) in Freund’s complete adjuvant (0.1 ml) intradermally. On day 21, animals were again treated with BTC-II along with booster incomplete Freund’s adjuvant injection. After 24 hrs, animal groups were administered intraperitoneally with their respective drug treatment, i.e., vehicle, BFTP (100, 200 and 400 mg/kg b.w.) and methotrexate (3 mg/kg b.w.), respectively once in a day for next 21 days. At the end of experiment serum level of pro-inflammatory cytokines was determined, and gut microbioata was analyzed by using Illumina HiSeq. Results Results revealed BFTP preadministered group exhibited significantly reduced level of paw swelling and arthritis score as compared to vehicle group. Inflammatory markers (IL-1β, IL-6, IL-7, IL-23 and TNF-α) of rheumatoid arthritis were also significantly alleviated by BFTP. Histological studies of mice ankle soft tissue also support the protective effect of BFTP. Additionally, BFTP repair altered gut microbial communities by reducing relative loads of inflammatory microbes such as Mucispirillum, Helicobacter and Lachnospiraceae. Conclusion The results conclude that BFTP possess beneficial anti-inflammatory response in arthritic model and can be used as a potential anti-arthritic agent for the management of arthiritis. Disclosure of Interests None declared