SAT0431\u2005RED GINSENG EXTRACTS SUPPRESS MONOSODIUM URATE CRYSTAL INDUCED NLRP3 ACTIVATION

Abstract

Background It is well known that red ginseng extracts (RGE) has anti-inflammatory properties. Objectives We aimed to investigate if RGE could suppress monosodium urate crystal (MSU) - induced NLRP3 activation and thus could be a potential therapeutic for gout. Methods Acute air-pouch model was used to investigate in vivo effect on acute gouty inflammation of RGE in mice. Human monocyte cell line –THP-1- was stimulated with MSU in the presence of RGE and expression of NLRP3, ASC, caspase-1, IL-1β was measured by PCR, immunoblotting and ELISA. Twenty four gout patients in intercritical period were randomized either to red ginseng tablet or placebo receiving group in a double-blind manner. After 3months of taking red ginseng tablets (RGT)/placebo, expression of NLRP3 and inflammatory cytokines of peripheral blood mononuclear cell was addressed by PCR. Results RGE sufficiently inhibited acute gouty inflammation in air-pouch mouse model, represented by reduced number of white blood cells in air pouch lavage fluid. In vitro, RGE dose-dependently suppressed MSU-induced IL-1 β production of THP-1 cells. RGE did not affect NLRP3 or pro IL-1 β expression. However, ASC oligomerization was inhibited and suppreseed NLRP3 inflammasome assembly. Patients taking RGT for 3 months showed significantly reduced NLRP3 expression compared to baseline, which was not observed in the control group. Conclusion RGE successfully suppressed MSU-induced acute inflammation in mouse model. Also, RGT reduced NLRP3 expression in intercritical gout patients. These results suggest that red ginseng can be implicated as a therapeutic agent for gout. References [1] Martinon F, Petrilli V, Mayor A, Tardivel A, Tschopp J. Gout-associated uric acid crystals activate the NALP3 inflammasome. Nature. 2006;440(7081):237-41. [2] 2 Kingsbury SR, Conaghan PG, McDermott MF. The role of the NLRP3 inflammasome in gout. Journal of inflammation research. 2011;4:39-49. Disclosure of Interests None declared