Abstract 5216: Identification of Sox2 regulatory factors in small cell lung cancer

Abstract

Sox2 is one of the core pluripotency genes that has also been implicated to be oncogenic in a multitude of cancers. We have shown that Sox2 is required for the formation of SCLC using mouse models and human cell culture. SCLC predominantly arises from in the lung from pulmonary neuroendocrine cells, which are formed from Sox2 -expressing progenitors that are then silenced upon terminal differentiation. However, upon transformation Sox2 becomes highly upregulated in SCLC. One means of repressing Sox2 is the Rb tumor suppressor, which binds to the Sox2 locus and represses transcription by the recruitment of many epigenetically silencing factors. However, there are still some gaps in our knowledge of the repression of Sox2 by Rb, such as which factors are recruiting Rb to Sox2 . We describe a novel technique of Cre-mediated Protein-DNA Immunoprecipitation (CrePI) to identify proteins at the Sox2 locus in an unbiased manner to further elucidate the mechanism of Rb repression of Sox2 . Citation Format: Hannah Wollenzien, Mira Yousef, Ellen Voigt, Michael S. Kareta. Identification of Sox2 regulatory factors in small cell lung cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 5216.