Nano Gold-Related PCR in Detection of MiR-145 and Its Correlation with Th1/Th2 in Premature Rupture of Fetal Membranes

Abstract

This study aimed to study miR-145’s correlation with Th1/Th2 in premature rupture of fetal membranes (pPROM) with nanogold-related PCR. Sixty pregnant women with pPROM between January 2019 to December 2019 were selected as the treatment group, and another sixty healthy pregnant\n women at the stage were chosen as the control group. Blood samples were collected for the quantification of miR-145 and the Th1/Th2 ratio. The result showed that nanoPCR could detect miR-145 with higher sensitivity and specificity. Then, nanoPCR detected a decreased miR-145 in pPROM pregnant\n women. Immunofluorescence staining and flow cytometry data presented a higher Th1/Th2 ratio in pPROM pregnant women. Western blotting showed the Th1/Th2 ratio increased significantly in pPROM pregnant women; also, the IFN-γ and IL-2 secreted by Th1 increased at the same time.\n On the other hand, the IL-4 and IL-6 secreted by Th2 were reduced, consistent with the change in Th2’s pattern in number reduction. A significant negative correlation was shown between Th1, Th1/Th2, and miR-145, and a positive correlation was shown between Th2 and miR-145. In conclusion,\n we propose thatin pPROM pregnant women, the decrease in miR-145 induces Th1 proliferation and Th2 reduction, causing relative cytokines to change and the immune system to lose imbalance. As more infections occur, and pPROM occurred as a result of fetal membrane fragility.