Does ophthalmic-grade silicone oil possess antimicrobial properties?

Abstract

PurposeTo test the antimicrobial properties of silicon oil (Aurosil 1000\xa0cSt, Aurosil Plus 5000\xa0cSt) on in vitro growth of common microorganisms causing endophthalmitis.Materials and methodsStaphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, multi-drug resistant (MDR) strain of Klebsiella pneumoniae, Escherichia coli, Candida albicans, and Aspergillus flavus were prepared to 0.5 McFarland turbidity. The bacteria and fungi were inoculated into the silicone oils, brain heart infusion (BHI) broth for bacteria and Sabouraud dextrose agar (SDA) broth for fungi, respectively, and cultured aerobically for 30\u2009days. From each sample, 10\u2009μl was plated onto nutrient agar and potato dextrose agar (PDA) for testing growth of bacteria and fungi respectively. Cultures from specimens, overnight incubation, and CFU counting were repeated on days 1, 3, 5, 7, 14, 21, 24, and 30. Negative controls were brain heart infusion and physiologic saline as well as silicone oils without any inoculations.ResultsAll bacteria showed a decrease in CFUs by the fifth day and eliminated between 21 and 30\u2009days in silicone oil. The silicon oil, irrespective of its viscosity, had only fungistatic effect up to 30\u2009days. Colony-forming units of microorganisms remained stable in physiologic saline during the study. In BHI and Sabouraud broth, both bacteria and fungi showed a growth pattern that was compatible with the growth curve of microorganisms.ConclusionMedical-grade silicone oil used in ophthalmology exhibited in vitro bactericidal and fungistatic activity over 30\u2009days. Insertion of silicone oil in vitrectomy for endophthalmitis, when required, could supplement the antimicrobial activities of intravitreal antibiotics in management of endophthalmitis.