[Overexpression of histone deacetylase 11 suppresses basal-like breast cancer cell invasion and metastasis].

Abstract

OBJECTIVE\nHistone deacetylase 11 (HDAC11) is a class Ⅳ member of histone deacetylase family, and its role in regulating cancer cell invasion and metastasis remains unclear. We aimed to investigate the role of HDAC11 in regulating the biological behaviors of basal-like breast cancer (BLBC) cells.\n\n\nMETHODS\nWe analyzed the expression of HDAC11 based on Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA). The effects of HDAC11 on the cell invasion and metastasis were examined using Transwell assay and in a mouse model. The interaction between HDAC11 and Twist was detected with immunoprecipitation. We identified HAS2 as a target gene of Twist using promoter luciferase assay and chromatin immunoprecipitation assay.\n\n\nRESULTS\nHDAC11 was lowly expressed in BLBC cells. HDAC11 overexpression suppressed BLBC cell invasion in vitro and their metastasis in nude mice. Mechanistically, HDAC11 directly interacted with Twist protein, antagonized its pro-invasive function and repressed Twist-induced HAS2 gene transcription.\n\n\nCONCLUSIONS\nOur data suggest that HDAC11 acts as a negative modulator of invasion and metastasis of BLBC cells.