Histochemical and Shoot Induction Studies of Basella rubra L . ( Basellaceae )

Abstract

The optimum concentrations of the plant hormones for in vitro regeneration and subsequent effect of auxins on rooting (in vitro and ex vitro) of shoots of Basella rubra L. have been investigated in present study. Nodal shoot segments were used as explants to initiate the cultures. Histochemical studies of in vitro and in vivo plants revealed alkaloids, polyphenols and terpenoids concentrations were higher in in vivo plants. Ascorbic acid and tannin concentration have shown no difference in the content in in vitro and in vivo plants. MS medium supplemented with IBA 2.0 mg/l + NAA 0.5 mg/l + GA3 0.5 mg/l induced shoot regeneration MS+IBA 3.0 mg/l induced roots and the plant regeneration was achieved in MS + IBA 0.5 mg/l + GA3 1.5 mg/l + IAA 1.0 mg/l. SEM – EDX elemental analysis of in vitro plants have shown absorption of sodium, magnesium, silica, phosphorus, sulphur, chlorine and potassium from the medium. The in vivo plants have shown absorption of magnesium, silica, chlorine, potassium, calcium and iron.