Synthesis of Novel Amide Functionalized Pyrido[2,3-d]pyrimidine\nDerivatives and their Anticancer Activity

Abstract

A series of novel amide functionalized pyrido[2,3-d]pyrimidine derivatives were synthesized from\n2-amino-6-(thiophen-2-yl)-4-(trifluoro-methyl)nicotinonitrile (1), which on reaction with trifluoroacetic\nacid to get pyridopyrimidine (2). Compound 2 when treated with bromoethyl acetate followed by\nhydrazine hydrate to get hydrazide derivatives 4. Compound 4 on reaction with different substituted\naromatic aldehydes to obtain Schiff base derivatives 5a-f. In another way, compound 3 treated with\ndifferent substituted amines and amino acids to obtain amide derivatives 6a-f, 7a-d and 8a-c. All the\ncompounds evaluated for anti-cancer activity against four human cancer cell lines such as A549-Lung\ncancer (CCL-185), MCF7-Breast cancer (HTB-22), DU145-prostate cancer (HTB-81) and\nHeLa-cervical cancer (CCL-2) and among the sythesized compounds, only 5d, 7d and 8a were identified\nas the potent compounds against the anticancer activity.