Effect of Aronia melanocarpa fruit juice on carrageenan-induced paw edema in metabolic syndrome rats

Abstract

INTRODUCTION: Chronic, low-grade inflammation plays a critical role in the pathogenesis of the metabolic syndrome (MS). Aronia melanocarpa fruits are rich in biologically active compounds—polyphenols, which possess a variety of health benefits including an anti-inflammatory effect. AIM: The aim of this article is to evaluate the effect of polyphenol-rich Aronia melanocarpa fruit juice (AMFJ) on carrageenan-induced acute inflammation in rats with diet-induced MS. MATERIALS AND METHODS: Forty male Wistar rats were included in the experiment. They were allocated into 4 groups: MS, MS+AMFJ 2.5 , MS+AMFJ 5 , and MS+AMFJ 10, all receiving high-fat high-fructose diet and 10% fructose in the drinking water for 10 weeks. The MS group served as a control and was treated daily with distilled water orally, while the other groups received AMFJ at doses of 2.5 mL/kg, 5 mL/kg, and 10 mL/kg, respectively. At the end of the experiment, carrageenan was injected in the left hind paw in order to induce acute inflammation. Paw edema was evaluated with plethysmometer on the 30 th min and 1 st , 2 nd , 3 rd , 4 th , and 5 th hour after the injection. RESULTS: In the MS group, the carrageenan-induced paw edema increased gradually with time reaching the highest value on the 5 th hour. A decrease throughout the whole 5-hour period was observed in groups treated with AMFJ, the effect being most pronounced and statistically significant in MS+AMFJ 5 group on the 2 nd and 3 rd hour. CONCLUSION: Aronia melanocarpa fruit juice treatment in rats with MS resulted in a decrease in the carrageenan-induced paw inflammation. The anti-inflammatory effect might be attributed to the polyphenols in AMFJ.