GDO ANALİZLERİNDE KULLANILAN CRM İZOLATLARININ KONTROLLÜ MUHAFAZA KOŞULLARINDA TEKRAR KULLANIM OLANAKLARININ ARAŞTIRILMASI

Abstract

Genetically modified organisms (GMOs) are used in many areas, especially in agricultural crops, due to their potential benefits. According to legal regulations, it is imperative to know the presence of GMOs in food and feed. Certified reference materials (CRM) are used in the accuracy of the analyzes, preparation of calibration curve, and in quality control studies. In this study, degradation occuring with freeze-thaw application has been investigated in order to determine the reuse possibilities of CRM isolates used in GMO analysis in herbal products. For this, freezing (-20 ± 2 ° C) and thawing (22 ± 2 ° C) processes were applied to the isolates obtained from soy and corn CRMs at certain periods (5, 10, 15, 20 times) under controlled conditions. Real time PCR analysis results showed that up to 20 freeze-thaw applications, CRM isolates were reusable.