Absence of RET/PTC1 rearrangement in a sample of Colombian individuals with papillary thyroid carcinoma

Abstract

espanolIntroduccion. El carcinoma papilar de tiroides es la neoplasia endocrina mas comun, por lo que se han evaluado marcadores con posible utilidad pronostica. Objetivo. Analizar la presencia del rearreglo del RET/PTC1, tiroiditis linfocitica y caracteristicas clinicas asociadas en pacientes con cancer papilar de tiroides en el Hospital de San Jose de Bogota D.C., Colombia. Materiales y metodos. Los casos con diagnostico de cancer papilar y tiroidectomia completa fueron seleccionados utilizando el registro de historias clinicas; el ARN se extrajo a partir del tejido tumoral y el ADNc se obtuvo utilizando una transcriptasa inversa para luego detectar el rearreglo del gen RET/PTC1 por medio de qPCR. Resultados. Se seleccionaron 55 pacientes con cancer papilar de tiroides; 93% correspondio a genero femenino, la edad promedio fue de 45.8 anos y la variante histologica mas frecuente fue la clasica (49%). Se evidencio una relacion entre tiroiditis linfocitica y la cantidad de ganglios positivos en segmentos distintos al vaciamiento central, asi como la tiroiditis y el valor de los anticuerpos antitiroideos. No se identifico la expresion del rearreglo RET/PTC1 en las muestras analizadas. Conclusiones. Se muestra una relacion entre tiroiditis linfocitica y la cantidad de ganglios positivos en segmentos distintos al vaciamiento central. Se debe continuar la busqueda de otros marcadores moleculares que permitan diferenciar el pronostico en estos pacientes. EnglishIntroduction: Papillary thyroid carcinoma is the most common endocrine neoplasm; therefore, markers with possible prognostic utility have been evaluated. Objective: To analyze the presence of RET/PTC1 rearrangement, lymphocytic thyroiditis and associated clinical features in patients with papillary thyroid cancer treated at the Hospital de San Jose in Bogota, Colombia. Materials and methods: Clinical records of patients with complete thyroidectomy and diagnosis of papillary cancer were retrospectively identified. RNA was extracted from tumor tissue, and cDNA was obtained using inverse transcriptase to detect the rearrangement of the RET/PTC1 gene by means of qPCR. Results: 55 patients with papillary thyroid cancer were selected; 93% were females, and the mean age was 45.8 years. The most frequent histological variant was classic (49%). A relationship was found between lymphocytic thyroiditis and the number of positive nodes in segments other than central draining, as well as thyroiditis and antithyroid antibody value. No RET/PTC1 rearrangement expression was found. Conclusions: A relationship between lymphocytic thyroiditis and the number of positive nodes in segments other than central draining was found. Other molecular markers should be searched to differentiate the prognosis of these patients.