МЕТАБОЛИЧЕСКАЯ АКТИВНОСТЬ НЕЙТРОФИЛОВ КРОВИ У БОЛЬНЫХ С ИНВАЗИЕЙ OPISTHORCHIS FELINEUS С КОЖНЫМ СИНДРОМОМ

Abstract

Aim: to study the functional activity of blood neutrophilic granulocytes in patients with invasion of Opisthorchis felineus with skin syndrome. Materials and methods. A total of 92 patients with chronic opisthorchiasis were examined, of which 38 patients (20 men and 18 women, average age 35.7±3.9 years) were with skin syndrome and 54 patients (28 men and 26 women, average age 36.5 ± 4.1 years) without skin syndrome, and 32 healthy patients (17 men and 15 women, average age 41.5 years). The main diagnostic method for opisthorchiasis was the determination of the eggs or bodies of adult parasites in the duodenal contents and/or feces, which was used in all 92 opisthorchiasis patients. Liver fibrosis was determined by the method of elastometry according to the METAVIR scale in all 92 opisthorchiasis patients. A study of the functional activity of neutrophilic granulocytes in the blood was carried out to all 92 patients with opisthorchiasis and 32 healthy individuals from the control group by chemiluminescent analysis with measurement of the intensity of the production of reactive oxygen species in spontaneous and zymosan-induced reactions in lucigenin and luminol-dependent processes. Results. Patients with opisthorchiasis with skin syndrome compared with patients with opisthorchiasis without skin syndrome have a higher incidence of pain in the right hypochondrium, dyspepsia, asthenovegetative syndrome, skin itching, blood eosinophilia, hyperbilirubinemia, cholestatic syndrome, cholecystitis, hepatomegaly, and also the content of immunoglobulin E. Liver fibrosis F3-F4 according to METAVIR was determined in 18.4% of opisthorchiasis patients with skin syndrome and in 11.1% of opisthorchiasis patients without skin syndrome (OR=1.78; CI 0.57-5.57; p=0 ,5). Both in luminol-dependent and lucigenin-dependent processes in opisthorchiasis patients with skin syndrome, a significant decrease in the functional activity of neutrophilic granulocytes was recorded in comparison with opisthorchiasis patients without skin syndrome, as evidenced by a significant decrease in the maximum intensity of reactive oxygen species production and the area under the chemiluminescence curve as in a spontaneous reaction, and in the zymosan-induced reaction. Conclusion. The results suggest that the presence of skin syndrome in patients with opisthorchiasis is accompanied by more significant clinical and laboratory manifestations, a decrease in the functional activity of blood neutrophils, and therefore it can be considered a marker of the severity of the pathology.The project “Immuno-biochemical model for predicting the severity of precancerous changes in the liver in patients with chronic opisthorchiasis” was funded by Krasnoyarsk Regional Fund of Science (application code: 2019051404996).