Salmonella Enteritidis forms biofilm under low temperatures on different food industry surfaces

Abstract

EnglishWe evaluated the influence of temperature on the ability of Salmonella Enteritidis (SE) to form biofilms on stainless steel, polyethylene, and polyurethane surfaces under different hygiene procedures. These materials were placed on SE culture and incubated at 42±1 oC, 36±1 oC, 25±1 oC, 9±1 oC, and 3±1 oC for 4, 8, 12, and 24 h. Hot water at 45 oC and 85 oC, 0.5% peracetic acid solution, and 1% quaternary ammonia were used for hygienization. Biofilm formation occurred at all temperatures evaluated, highlighting at 3 oC which has not been reported as an ideal temperature for the adhesion of SE to these materials. The SE adhered more often to polyethylene surfaces than to polyurethane and stainless steel surfaces (P portuguesAvaliou-se o efeito da temperatura na capacidade de Salmonella Enteritidis (SE) formar biofilme em superficies de aco inoxidavel, polietileno e poliuretano e diferentes processos de higienizacao. Corpos de prova destes materiais foram postos frente a culturas de SE e incubados a 42±1 oC, 36±1 oC, 25±1 oC, 9±1 oC e 3±1 oC por 4, 8, 12 e 24 horas. Para a higienizacao foram testados agua aquecida a 45oC e 85 oC e solucoes de acido peracetico 0,5% e amonia quaternaria 1%. Verificou-se a formacao de biofilmes em todas as temperaturas avaliadas, ressaltando-se a 3 oC, ainda nao citada como propicia para adesao de SE. Houve maior adesao ao polietileno do que ao poliuretano e ao aco inoxidavel (P