Identificación de helicobacter pylori a partir de lesión aterosclerótica coronaria humana

Abstract

Introduction: The main pathogenic mechanism underlying the genesis and development of diseases of the circulatory system is atherosclerosis. It is a complex and progressive pathological process of the arterial wall that affects especially the coronary, cerebral and peripheral arteries. Currently, there is talk about The infectious hypothesis of atherosclerosis . Helicobacter pylori infection has been one of the most researched worldwide. Objective: To identify H. pylori from atherosclerotic lesions of patients who attend the cardiovascular and angiology services of the city of Barranquilla. Methods: A descriptive cross-sectional study was carried out. In a sample of 102 participants. The atheromas were taken by expert personnel in endarterectomy and dissection of blood vessels. The specimens were embedded in 4% formaldehyde solution. DNA extraction was performed from atheromas. The molecular diagnosis of H. pylori was performed by ANIDADA PCR, evidencing a 120 bp fragment after electrophoresis in a 3.5% agarose gel in 0.5X TBE. Results: 102 samples of atheromas were obtained. One sample was positive for H. pylori (Sample # 14, 1/102). The atherosclerotic tissue was obtained from the right coronary artery. Conclusion: The infectious hypothesis of atherosclerosis has been one of the main research topics worldwide in recent decades. H. pylori infection is a risk factor, however, several studies are necessary to be able to conclude more accurately.