Journal of Science, Natural Science | 2021
Identification and analysis of stress-assiciated protein (SAP) encoding genes in papaya (Carica papaya L.) by using in silico methods
Abstract
By using the in silico methods, total of seven stress-associated protein encoding genes have identified in the geneome of papaya (Carica papaya L.). The full-length genomic sequence of papaya SAP genes were ranging from 416 to 813 nucleotides. These genes had single intron or were intronless. The predicted protein sequences included from 114 to 270 amino acids, according to the molecular weight ranged from 13.10 to 29.63 kDa. These proteins were alkaline with a pI value ranging from 8.82 to 9.95. Based on the protein structure and the phylogeneic analysis, the papaya SAP were divided into two groups, I (five members) and II (two members). The papaya SAP had a highly conserved level of structure including two conserved regions, A20-AN21 (group I), or AN21 domains only (group II). RNA-seq analysis showed that all of seven SAP genes expressed in papaya leaves and were induced by freeze thaw awakening treatment (in comparison with control treatment). CpSAP2 showed the highest relative expression level in compared to other SAP genes of papaya. The results of this work have an important significance and are base of the further research on gene cloning, functional analysis of SAP genes and breeding of papaya in response to environmental abiotic stresses.