Indian Journal of Obstetrics and Gynecology Research | 2019
Impact of TLR9 gene polymorphism on cervical HPV infection and its progression to cervical cancer
Abstract
Introduction: Cervical carcinogenesis is a multi-step process associated with refractory infection by\nhigh-risk human papillomavirus (HPV) types. Only a minority of HPV infected women develop cervical\nintraepithelial neoplasia (CIN) or cervical cancer, indicating that HPV infection is not the sole risk factor to\ninduce cervical cancer.. Single nucleotide polymorphisms are the most common form of genetic variants in\nhuman genome, some of which can influence the susceptibility to human diseases including cancer. TLR9\ngene polymorphisms appear to have considerable role in disease susceptibility, including HPV induced\ncervical cancer.\nAim: The present study aims to identify the role of TLR9 C2848T (rs352140) gene polymorphism in\ncervical cancer susceptibility in East Indian women.\nMaterials and Methods: Our study was a case – control study. Study subjects comprised 33 women with\nhistologically proven cervical cancer, and 24 women with benign cervical lesions. The case group included\nHPV 16 +ve subjects with malignancy. Among the benign group, 15 were HPV16 +ve (intermediate group)\nand 9 were HPV –ve (control group).. HPV status and HPV type was confirmed by PCR based method,\nusing specific primers. TLR9 genotyping was performed using PCR RFLP. TLR9 expression was analyzed\nacross all categories of samples using real time PCR.\nResults: Our study showed increased expression of TLR9 in malignant group, compared to the control\ngroup. TLR9 expression was also increased in the HPV +ve nonmalignant group, compared to HPV –ve\ncontrols. Correlating TT, CT, CC genotype with TLR9 expression analysis across malignant group and\ncontrol group, there was increased expression of TLR9 among TT genotype and CT genotype compared to\nCC genotype.\nConclusion: Our study showed that TLR9 C2248T polymorphism causes upregulation of TLR9 expression\namong cervical cancer patients. Our studies suggest that the TLR9 C2848T (rs352140) polymorphism may\nbe a risk factor of cervical cancer in East