Effect of Foliar Application of Methanol on Changes of Antioxidant Enzymes of Vigna unguiculata L. in Water-Deficit Stress

Abstract

The aim of this study was to investigate the effect of foliar application of methanol on the activity of antioxidant enzymes of Vigna unguiculata L. in water-deficit stress conditions. The trials were performed in a split plot design arranged in completely randomized block design with three replications in two years cultivation (2017 and 2018). Water-deficit stress treatments were considered in the main plots in four levels (25, 50, 75 and 100% of plant water requirement) and combined foliar application of methanol treatment in three levels (control, 10 and 20% vol.), and genotype treatment in two cultivars of Omidbakhsh 1057 and Mashhad in sub plots. The results showed that the effect of water-deficit stress and foliar application of methanol on the activity of antioxidant enzymes were significant (P £ 0.01). The effect of genotype on the activity of ascorbate peroxidase was just significant (P £ 0.01).