Molecular Characterization of the TLR2 Gene in Datong Yak

Abstract

The coding region of Datong yak’s TLR2 gene was amplified and subjected to sequence characterization. The coding region of the Datong yak TLR2 gene comprised a single ORF of 2355 nucleotides that coded for 784 amino acids with translatable products. The coding region of the TLR2 gene of the Datong yak contained two nucleotide mutation sites, namely, G677A and G1587A. G677A exhibited a missense mutation. After comparing nucleotide and amino acid sequences among related species and constructing the phylogenetic relationships, Datong yak sequences were shown to be highly similar to those of Bos taurus. The Datong yak TLR2 protein simultaneously possessed leucine-rich repeats, a TIR domain and an aldehyde dehydrogenase active site. Results showed that the protein plays an important role in the body’s immune regulation mechanism.