International Journal of Current Microbiology and Applied Sciences | 2019
In vitro Shoot Proliferation from Nodal Segments of Indian Blackberry (Syzygium cumini L.)
Abstract
India is a tropical country, where many minor fruit tree species are very popular and their fruits are always in high demand as the seasonal delicacies. However, most of these fruit trees are not commercially cultivated due to the poor viability of their seeds and remain underutilized for their medicinal and nutritional value. These fruit trees provide a significant support to the livelihood of rural and tribal people as they generate additional household income. Indian Blackberry (Syzygium cumini L.) locally known as ‘Jamun’ is one of such underutilized minor fruit species having great importance in India. The use of medicinal plants plays an important role in the lives of rural poor peoples, Micropropagation of Indian Blackberry (Syzygium cumini L.) species locally called as Jamun, has always been a challenging task. In India, Jamun tree is an underutilized minor fruit crop species possessing a high medicinal and nutritional value. An efficient protocol for rapid shoot proliferation of Jamun (cv. Rajamun) from nodal segment of locally grown mature trees has been developed. Experiments were conducted at Biotech Centre, Dr. PDKV, Akola during 2016-18. The Lloyd and McCown woody plant medium (WPM) used as a basal media supplemented with growth regulators at varying concentrations. Nodal explants were cultured on WPM supplemented with BAP (6-Benzylamino purine 8, 8.5 and 9 μM/l), Kn (Kn 8, 8.5 and 9 μM/l). WPM supplemented with BAP + Kn @ 8 μM/l was proved to be significantly best at 5% level for high survival rate of 93.33% and required less time i.e. 32.43 days to initial shoot sprout after explants inoculated with 2.53 initial shoot multiples. The treatments for shoot proliferation were conducted on WPM supplemented with BAP @ 13, 14 and 15 μM/l, Kn @ 8, 8.5 and 9 μM/l and NAA (Naphthalene acetic acid) @ 5 μM/l. For high frequency multiple shoot induction WPM with 13μM BAP + 8.5μM Kn + 5μM NAA was proved to be significantly superior at 5% level after 4 weeks of first subculture giving 2.87 shoot multiples, followed by maximum 4.20 shoot multiples at 6 weeks stage of second subculture with significantly 3.21cm shoot length elongation at 10 weeks when supplemented on same media against 1 cm of control. K e y w o r d s