Establishing Differentiated Air-Liquid Interface Primary Human Bronchial Epithelial Cell Cultures

Abstract

\n This protocol describes the establishment and maintenance of differentiated primary human bronchial epithelial cell (pHBEC) cultures from primary bronchial epithelial cells using Lonza and Gibco media. Note: This is a historical protocol. At the time of publication, this protocol has been superseded by a different version in the McCullough lab; however, it is being published to support the transparency and reproducibility of other studies by which it is referenced.Disclaimer: The information presented here has been reviewed and approved for publication by the US Environmental Protection Agency do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.