Benefit Clarification of Longxuetongluo Capsule Towards Ischemic Stroke Rats by ischemic stroke rats by LC MS/MS Based Metabolomics

Abstract

\n Background:Longxuetongluo Capsule (LTC) that was prepared from the phenolic compound cluster of Chinese dragon’s blood, has been clinically utilized for years towards ischemic stroke. However, its therapeutic mechanism s remain unclear. The present study aimed to evaluate the therapeutic effect and to explore the underlying mechanisms of LTC on ischemic stroke rats characterized by cerebral ischemia 6 re perfusion injury. Method:Ischemic stroke rats were simulated by middle cerebral artery occlusion ( and validated by the assays in terms of histological and behavioral assessments . The plasma metabolome comparisons amongst different groups were conducted by UHPLC Q Executive MS in combination with subsequent multivariate statistical analysis , aiming to finding the molecules in respond to the surgery or LTC treatment . Result:Intragastric administration of LTC significantly decreased not only the neurobehavioral abnormality scores but also the cerebral infarct area of MCAO rats. A total of 21 potential biomarkers related to ischemic stroke were observed, and 18 biomarkers (such as LysoPC, PE, SM, N1-Methyl-4-pyridone-3-carboxamide, PE (24:1/18:3 etc.) were selected after Youden index >0.7. The results showed that LTC was beneficial to ischemic stroke rats induced by ischemia reperfusion injury by Glycerophospholipid metabolism , Glycosylphosphatidylinositol (GPI) anchor biosynthesis , Nicotinate and nicotinamide metabolism and Sphingolipid metabolism Conclusion:The present study could provide evidence that metabolomics, as system systematicatic approach, revealed its capacity to evaluate tapproach, revealed its capacity to evaluate the holistic efficacy of TCM, he holistic efficacy of TCM, and investigate the molecular mechanism underlying the clinical treatment of LTC on and investigate the molecular mechanism underlying the clinical treatment of LTC on ischemic stroke. ischemic stroke.