Antioxidant metabolites from riparian fungal endophytes improve the tolerance of rice seedlings to flooding

Abstract

\n Endophytic fungi have the potential to enhance plant resistance to various stresses and promote the ecological adaptation of the hosts. To evaluate the effects of the riparian endophytes on rice seedlings to flooding tolerance, here we screened out two endophytes from the plant Myricaria laxiflora growing in the Yangtze River zone. Through morphological characteristics and rDNA ITS (internal transcriber region) sequence, the two strains were respectively identified as Aspergillus fumigatus and Chaetomium globosum. Metabolites derived from both fungi were capable of increasing tolerance to flooding. Systematic separation and purification coupled to bioassays revealed that two natural antioxidants, Z-N-4-hydroxystyryl formamide (NFA) and chaetoglobosin A (CheA), were effective for alleviating flooding stress. Both NFA and CheA inhibited the production of factors such as malondialdehyde, superoxide dismutase, ethanol dehydrogenase, and NADPH oxidase that are associated with oxidative stress induced by flooding. Gene expression analyses of NADPH oxidase families indicated that OsRbohB could be involved in conferring flooding tolerance mediated by the two natural antioxidants. These findings contribute to understanding the role of the natural antioxidants in riparian endophytic fungi, and providing a basis for improvement of flooding tolerance of rice and other crop plants through the antioxidants of the fungal endophytes.